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© Research
Publication : Nature communications

A microfluidic platform for highly parallel bite by bite profiling of mosquito-borne pathogen transmission.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nature communications - 14 Oct 2021

Kumar S, Hol FJH, Pujhari S, Ellington C, Narayanan HV, Li H, Rasgon JL, Prakash M,

Link to Pubmed [PMID] – 34650045

Link to DOI – 10.1038/s41467-021-26300-0

Nat Commun 2021 10; 12(1): 6018

Mosquito bites transmit a number of pathogens via salivary droplets deposited during blood-feeding, resulting in potentially fatal diseases. Little is known about the genomic content of these nanodroplets, including the transmission dynamics of live pathogens. Here we introduce Vectorchip, a low-cost, scalable microfluidic platform enabling high-throughput molecular interrogation of individual mosquito bites. We introduce an ultra-thin PDMS membrane which acts as a biting interface to arrays of micro-wells. Freely-behaving mosquitoes deposit saliva droplets by biting into these micro-wells. By modulating membrane thickness, we observe species-dependent differences in mosquito biting capacity, utilizable for selective sample collection. We demonstrate RT-PCR and focus-forming assays on-chip to detect mosquito DNA, Zika virus RNA, as well as quantify infectious Mayaro virus particles transmitted from single mosquito bites. The Vectorchip presents a promising approach for single-bite-resolution laboratory and field characterization of vector-pathogen communities, and could serve as a powerful early warning sentinel for mosquito-borne diseases.

https://pubmed.ncbi.nlm.nih.gov/34650045