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© Research
Publication : SPARS'09 - Signal Processing with Adaptive Sparse Structured Representations

A Compressed Sensing Approach for Biological Microscopy Image Denoising

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in SPARS'09 - Signal Processing with Adaptive Sparse Structured Representations - 09 Mar 2009

Marcio Marim, Elsa Angelini and Jean-Christophe Olivo-Marin

SPARS'09 - Signal Processing with Adaptive Sparse Structured Representations, 2009

Compressed Sensing (CS) provides a new framework for signal sampling, exploiting redundancy and sparsity in incoherent bases. For images with homogeneous objects and background, CS provides an optimal reconstruction framework from a set of random projections in the Fourier domain, while constraining bounded variations in the spatial domain. In this paper, we propose a CS-based method to simultaneously acquire and denoise data based on statistical properties of the CS optimality, signal modeling and characteristics of noise reconstruction. Our approach has several advantages over traditional denoising methods, since it can under-sample, recover and denoise images simultaneously. We demonstrate with simulated and practical experiments on fluorescence images that we obtain images with similar or increased SNR even with reduced exposure times. Such results open the gate to new mathematical imaging protocols, offering the opportunity to reduce exposure time along with photo-toxicity and photo-bleaching and assist biological applications relying on fluorescence microscopy.