Birth date : 27/5/1956
Nationality : French
- 1988 : EPHE “Réponses immunitaire et idiotypie” – Sorbonne I University – Paris
- 1982 : DUT A “Immunologie” – Necker, Pasteur – Paris
- 1981 : BTS Analyses Biologiques – ESTBA – Paris
- 1975 : Bac D – Lycée F. Schmidt – St Cloud
- 2003 : Engineer II – Institut Pasteur – Paris
- 1998 : Engineer I – Institut Pasteur – Paris
PROFESSIONAL EXPERIENCES & TECHNICAL SKILLS :
2012-2015 : “Studies on thymic precursors in the mouse embryo, the two waves of settling progenitors. Study of haematopoietic stem cells, fetal liver stroma cells and bone marrow stem cells. Analysis of circulation, multiplication and migration of intraepithelial lymphocytes”.
Techniques : Isolation of epithelial cells. Multiple organotypic cultures, PCR, thymectomies, grafts, intrasplenic injections. Cell sorting of precursors, intra-embryonic injections. Biomark. Thoracic duct cannulation. Lymphopoiesis unit – Institut Pasteur – Paris
2009-2011 : “Repertoire’studies of regulatory T cells in adult and in the newborn mouse. Conversion phenomena in deficient mice. Analysis of fetal liver progenitors. Study of Notch expression in embryogenesis”.
Techniques : Immunoscope I et II and sequencing. Qualitative and quantitative PCR, cytometry, cycle analysis, organotypic cultures, thymectomies, grafts. cell sorting, intra-embryonic injections. Lymphopoiesis unit – Institut Pasteur – Paris
2002-2008 : “Foxp3+ T cells in the newborn mouse. Ontogeny and settling of the first natural Regulatory T cells. Repertoire analysis of regulatory T cells”.
Techniques : Molecular biology, sequences, Immunoscope I et II. Quantitative PCR, intra-cytoplasmic stainings, cellular cycle analysis, intrathymic injections in the adult and in the newborn mouse, intra-femoral injections, organotypic cultures, quantitative PCR, sequencing, grafts under the kidney capsula, in vitro stimulations. Lymphopoiesis unit – Institut Pasteur – Paris
1998-2001 : “Regulatory T cells in inflammatory bowel disease. Ontogeny and physiology of natural Regulatory T cells and their functions”.
Techniques : Flow Cytométry 6 colors. Adult mouse thymectomy and multiple mouse injections. Lymphocyte Development unit – Institut Pasteur – Paris
1990-1997 : “Immune Tolerance, Tolerance analysis and properties of intraepithelial lymphocytes (IEL)”.
Techniques : Isolation of intestinal lymphocytes. Surgery techniques and skin grafts, Flow cytometry, fundamental molecular biology, protein purifications, In Situ Hybridization, cloning. Immunobiology unit – Institut Pasteur – Paris
1990 : Student at FEBS international summer school – Greece
1982-1989 : “Analysis of natural antibody network. Idiotypes’ network. Natural antibodies’repertoire in the newborn mouse”. Techniques : Cellular fusion techniques, ELISA. Culture assays. Immunobiology unit – Institut Pasteur – Paris