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© Research
Member: Assistant Professor - Alumni

Negar Seyed

Scientific Fields
Diseases
Organisms
Applications
Technique
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Connections

CV

Education :

B.Sc. (1996-1998): Cellular and Molecular Biology, Faculty of Science, Tehran University, Tehran, Iran

M.Sc.(1999-2002) : Medical Immunology, Faculty of Medicine, Shahid Beheshti Medical School, Tehran, Iran

Ph.D. (2006-2012): Medical Biotechnology, Pasteur Institute of Iran, Tehran, Iran

 

Experiences :

Research assistant of Cellular and Molecular Research Center, Faculty of medicine, Shahid Beheshti Medical School .2002 _ 2006

Sabbatical opportunity: Besancon France, UMR 1098, Sep 2011-May 2012

 

Position :

 

Assistant professor at Pasteur Institute of Iran, Immunotherapy and Leishmania Vaccine Research Dept. Jan 2013 onward

 

Area of Expertise :

Reverse Vaccinology and whole genome mining for new vaccine candidates

Peptide Vaccine Research for Leishmanisis

Sub-unit vaccine development focusing on adaptive immune correlates that control early inflammatory conditions by innate immunity especially neutrophils.

 

Teaching experience :

 

Teaching         courses    and     workshop    holding     on     Reverse     vaccinology     and Immunoinformatics at Pasteur Institute of Iran, since 2013.

Workshop holding on “Immunoinformatics and vaccine design”, 12th International Congress of Immunology and Allergy, April-May, 2014.

Workshop holding on “Basic flow cytometry and applications”, Feb. 2016 and Feb 2017.

Workshop holding on “ELISA basics and set-up methods”, Feb 2016.

“Vaccine development against vector borne diseases”, Cell therapy, Gene Therapy and Immunotherapy course for Ph.D students, November 2017.

DNA vaccines: novel approaches, Cell therapy, Gene Therapy and Immunotherapy course for Ph.D students, November 2017.

“Lipid mediators in inflammation and resolution”, Advanced Cellular and Molecular Course for Ph.D students, May 2018.

 

Research grants :

Design of a DNA construct encoding in silico predicted Leishmania major specific human CD8 T cell stimulating epitopes and evaluation in HLA transgenic mice, Iran National Science Foundation, Grant number 87020176 (Ph.D thesis).

Assessment of induced CD8+ T cells following DNA and Live vaccine using Leishmania encoding MHC class I restricted epitopes at pre and post challenge against L. major in BALB/c   mice model.   Pasteur Institute of Iran, Grant number: 663 (Principal investigator).

Evaluation of protection induced against Leishmania major by in-vitro maturated MoDCs presenting CD8 stimulating peptides after a heterologous vaccination regimens as DC- Prime/DNA-Boost in BALB/c mice model. Pasteur Institute of Iran, Grant number: 956 (Principal investigator).

Human correlates of concomitant Immunity against cutaneous leishmaniasis due to Leishmania major   in Asymptomatic and Healed Individuals. LeiSHield-MATI international project (Principal Investigator).

Evaluation of therapeutic effects of DNA and Live Leishmania encoding human HNP-1 against cutaneous leishmaniasis in Balb/c mice, Pasteur Institute of Iran, Grant number 705 (collaborator).

Cloning and expression of Phlebotomus papatasi SP15 protein in Lactococcus lactis and evaluation of its protective effect cutaneous leishmaniasis in Balb/c model, Pasteur Institute of Iran, Grant number: 1032 (collaborator).

Identification of Phlebotomus sergenti salivary gland immunogenic proteins and evaluation of their protective role against L. tropica induced cutaneous leishmaniasis in Balb/c model, Pasteur Institute of Iran, Grant number: 756 (collaborator).

 

Editorial Board member :

Journal of “Medical Microbiology and Infectious Diseases” since 2016, published in Pasteur Institute of Iran

 

Scientific Curriculum :

Attending first International Workshop of Leishmani, Pasteur Institute of Iran, March 2008, Tehran, Iran

Attending 4th World Congress on Leishmaniasis, Worldleish4, Feb 2009, Lakno, India.

Poster presentation at 14th International Congress of Immunology, Aug 2010, Kobe, Japan.

Attending Scientific Meeting of the Young researchers of RIIP Paris, November 2011, Paris, France.

Oral presentation at 5th World Congress on Leishmaniasis, Worlleish5, May 2013, Porto de Galinhas, Brazil.

Oral presentation at Anual Scientific Meeting of researchers of Pasteur Institute International Network, Paris, Sep 2014.

Attending EMBO conference “Microbiology after Genomics revolution” (Genome 2014) Pasteur Institute of Paris, June 2014.

Attending theoretical and practical training course in “Metabolomics and it’s data analysis in Systems Biology”, Pasteur Institute of Iran, January 2015.

Poster presentation at Annual Scientific Meeting of researchers of RIIP Paris, Oct 2015.

Attending MOOC vaccinology course, Pasteur Institute of Paris, Sep 2015.

Collaborating in the organization of the second workshop on Leishmania and Leishmaniasis, Pasteur Institute of Iran, Tehran, Iran, Nov 2016,

Contributing in MATI regional meeting of “Pasteur Institute International Network”, Tehran, Iran, Nov 2016.

Attending annual Scientific Meeting of researchers of Pasteur Institute International Network, Paris, Nov-Dec, 2016.

Attending “Innate Immunity and Infectious Diseases” course, Pasteur Institute of Paris, April 2017, Paris, France.

Poster presentation at 6th World Congress on Leishmaniasis, Worlleish6, May 2017, Toledo, Spain

Contributing in LeiSHIeld- MATI: A multi-disciplinary international effort to identify clinical, molecular and social factors impacting cutaneous leishmaniasisCall H2020- MSCA-RISE-2017.

 

Technical expertise and computational knowledge :

Cell culture

Flow cytometry (intra cellular cytokine staining)

Immunoassays including cytotoxicity assays, ELISpot, ICCS, CFSE, ELISA

PCR and electrophoresis

Gene cloning

Western blotting

Immunoinformatics and in silico peptide prediction

BMDC differentiation and maturation

DNA vaccine

 

Publications :

Seyed, NC. Peters, S. Rafati, “Translating Observations from Leishmanization into non-Living Vaccines: The Potential of Dendritic Cell Based Vaccination Strategies against Leishmania”, 2018, Frontiers in Immunology, 9:article1227.

Abdossamadi, T. Taheri, N. Seyed, H. Montakhab-Yeganeh, F. Zahedifard, “Live Leishmania tarentolae secreting HNP1 as an immunotherapeutic tool   against Leishmania infection in BALB/c mice”, 2017, Immunotherapy, 9 (13): 1089-1102.

Abdossamadi, N. Seyed, F. Zahedifard, T .Taheri, Y. Taslimi, “Human Neutrophil Peptide 1 as immunotherapeutic agent against Leishmania infected BALB/c mice”, 2017, PLoS neglected tropical diseases, 11 (12), e0006123.

Seyed, S. Rafati. “Innate Immunity Plays a Key Role in Leishmania Infection: Implications for Vaccine Design”, Journal of Medical Microbiology and Infectious Diseases, 2016, 4 (3): 39-44.

T. Taheri, Seyed, S Rafati, DNA Integration in Leishmania Genome: An Application for Vaccine Development and Drug Screening, 2016, Vaccine Design: Methods and Protocols: Volume 1: Vaccines for Human Diseases, pages 603-622.

T. Taheri, Seyed, A. Mizbani, S. Rafati. “Leishmania-based expression systems”, Applied microbiology and biotechnology, 2016, 100 (17): 7377-7385.

Abdossamadi, N. Seyed, S. Rafati. “Mammalian host defense peptides and their implication on combating Leishmania infection”, Cellular immunology, 2016, 309: 23-31.

Sadeghi, N. Seyed, S. Rafati, T. Taheri, “Optimization of the Timing of Induction for the Assessment of Nitric Oxide Production in Leishmania major Infected Macrophage Cells”, Iranian journal of parasitology, 2016, 11 (3): 325

Seyed, T. Taheri, S. Rafati. “Post Genomics and Vaccine Improvement for Leishmania”. Front Microbiol. 2016, 7: 467.

T. Taheri, Seyed, A. Mizbani, S. Rafati. “Leishmania-based expression systems”. Appl Microbiol Biotechnol, 2016, 100 (17), 7377-85.

Abdossamadi, N. Seyed. S. Rafati. “Mammalian host defense peptides and their implication on combating Leishmania infection”. Cellular Immunology, 2016, 309:23-31.

M. Zandieh, T. Kashi, T. Taheri, Zahedifard, Y. Taslimi, F. Doustdari, S. Habibzadeh, A. Eslamifar, F. Shokri, S. Rafati, N. Seyed. “Assessment of Protection Induced by DNA and Live Vaccine Encoding Leishmania MHC Class I Restricted Epitopes against L. major Challenge in Balb/c Mice Model”. J Microbial and Biochemical Technology, 2015 7: 427-438.

Sadeghi, N. Seyed, M.H. Etemadzadeh,S. Abediankenari, S. Rafati,T. Taheri. “In Vitro Infectivity Assessment by Drug Susceptibility Comparison of Recombinant Leishmania major Expressing Enhanced Green Fluorescent Protein or EGFP-

S. Jamshidi,   M. Hashemi,   S. Rafati,   T. Taheri.   “EGFP   reporter   protein:   its immunogenicity in Leishmania-infected BALB/c mice”. Applied Microbiology and Biotechnology, 2015:1-

T. Taheri, S. Nik, N. Seyed, F. Doustdari, M.H. Etemadzadeh, F. Torkashvand, S. Rafati. “Generation of stable L. major+ EGFP-LUC and simultaneous comparison between EGFP and luciferase sensitivity”. Experimental parasitology, 2015,150, 44-55

Seyed, T. Taheri, C. Vauchy, M. Dosset, Y. Godet, A. Eslamifar, I. Sharifi, O. Adotevi, C. Borg, P. S Rohrlich, S. Rafati. “Immunogenicity Evaluation of a Rationally Designed Polytope Construct Encoding HLA-A*0201 Restricted Epitopes Derived From Leishmania major Related Proteins in HLA-A2/DR1 Transgenic Mice: Steps Toward Polytope Vaccine.” PLoS ONE, 2014, 9(10), e108848.

T. Taheri, Gholami, F. Saatchi, N. Seyed, Y. Taslimi, S. Rafati “Expressional comparison between Episomal and stable transfection of a selected tri-fusion protein in Leishmania tarentolae”. Vaccine Research, 2014, 1(1): 1-9

Seyed, T. Taheri, C. Vauchy, M. Dosset, I. Sharifi, P. S. Rohrlich, S. Rafati. “Rationally designed DNA construct encoding MHC class I restricted epitopes derived from Leishmania major proteins successfully provokes Cytotoxic CD8 T cell responses in Balb/c mice.” Front. Immunol. Confrence abstract: 15th international congress of immunology. Doi: 10.3389/conf.fimmu.2013.02.00987

Bandehpour, Z. Sharifnia, Mohajeri, M. Taherkhani, N. Seyed, A. Koochaki, F.Yarian, R. Shirvani, P.Pakzad, H. Saadat, B. Kazemi. “Comparative study of the reactivity of natural and mutated streptokinase with total anti-streptokinase Abs in human sera”. Blood Coagolation and Fibrinolysis. Blood Coagulation & Fibrinolysis: 2012, 23(8):734-738

 

Seyed, F. Zahedifard, Sh. Safaiyan, E. Gholami, F. Doustdari, K. Azadmanesh, M. Mirzaei, N.Saeedi Eslami, A. Khadem Sadegh, A. Eslamifar, I. Sharifi, S. Rafati. “In Silico Analysis of Six Known Leishmania major Antigens and In Vitro Evaluation of Specific Epitopes Eliciting HLA-A2 Restricted CD8 T Cell Response”. PLoS NTD. 2011. 5 (9): e1295. parasitology 2011; 128(1):9-17.

Bolhassani, T. Taheri, Y. Taslimi, S. Zamanilui, F. Zahedifard, N. Seyed, F. Torkashvand, B. Vaziri, S. Rafati. “Fluorescent Leishmania species: development of

stable GFP expression and its application for in vitro and in vivo studies”. Exp Parasitol. 2011, 127 (3):637-4.

M. Salehi, A. Bolhassani, T. Taheri, Mohit, N. Seyed, F. Zahedifard, Y. Taslimi, M.

Sattari and S. Rafati. “Construction of a recombinant Leishmania tarentolae expressing human papillomavirus type 16 E7 gene and evaluation of its immunogenicity in C57BL/6 mice model”. Clin Biochem, 2011. 44 (13):S74.

Kazemi, N. Seyed, E. Moslemi, M. Bandehpour, M. Bikhof Torbati, N. Saadat, A. Eidi, E.Ghayoor, F. Azizi, “Insulin receptor gene mutations in Iranian patients with type II Diabetes Mellitus”, Iranian Biomedical Journal, July 2009,13 (3): 161-168.

Kazemi, N. Seyed, P. Shekari, M. Bandehpour, Z. Sharifnia, K. Parivar . “Production of mutant recombinant streptokinase protein”. Archives of Clinical Infectious Diseases, 2008. 3 (4). 179-183.

B. Kazemi, seyed, M. Bandehpour, P. pakzad, “Cloning, Expression, and purification of Truncated Chlamydia Trachomatis Outer membrane Protein 2 (Omp2) and its application in an ELISA assay”, Iranian J. Immunology. Sep 2008, 5(3), 148-155.

B. Kazemi, Fallahian, N. Seyed. “Molecular cloning of the streptokinase mutant gene”. Pakistanian Journal of Biological Sciences, 2006, Vol. 9, No.3: 546-8.

M. Bandehpour, Seyed, M. Shadnoush, P. Pakzad, B. Kazemi, “Using recombinant Chlamydia Major Outer Membrane Protein (MOMP) in ELISA diagnostic Kit” ,Iranian Journal of Biotechnology, 2006, Vol. 4, No. 4: 239-244.

Saadat, Pakzad, P. Seyed, N. “Assesment of Anti-Streptokinase Antibody in Patients with Heart Diseases and Normal Subjects”. Iranian Journal of Immunology, 2004, Vol. 1, No. 1: 63-70.

Kazemi, Yasaee, F, Bandehpour, M. Seyed N. “Diagnosis of Trichomonas Vaginalis Infection by Urine PCR Analysis Compared to wet mount Microscopic Screening”. Journal of Medical Sciences, 2004, Vol. 4, No. 3:206 – 9.

NCBI submissions

Homo sapiens isolate 116 insulin receptor gene, exon 9 and partial cds gi|145554253|gb|EF207612.1|[145554253]

Homo sapiens isolate 121 insulin receptor gene, exon 13 and partial cds gi|145554249|gb|EF207609.1|[145554249]