The research group of the UBI studies membrane assembly of viruses, focusing predominantly on large DNA viruses. Using tools collected over the years, we use virus-infected cells to develop advanced EM-embedding and imaging methods that can be applied by the users of our platform. We put a particular emphasis on the development on two aspects in EM. The development of high-resolution cryo-EM methods to determine the structure of viral membrane-associated proteins in vitro and in situ. We also wish to broaden our portfolio of correlated- light and electron microscopy methods. For both we develop workflows that involve sample preparation, semi-automated imaging and image processing. We particularly welcome the input of autonomous users that can complement our efforts along their biological questions.
Chlanda, P., Carbajal, M.A., Cyrklaff, M., Griffiths, G., and Krijnse Locker, J. (2009). Membrane rupture generates single open membrane sheets during vaccinia virus assembly. Cell Host & Microbe 6, 81-90.
Chlanda, P., and Krijnse Locker, J. (2017). The sleeping beauty kissed awake: new methods in electron microscopy to study cellular membranes. Biochem J 474, 1041-1053.
Krijnse Locker, J., Chlanda, P., Sachenheimer, T., and Brügger, B. (2013). poxvirus membrane biogenesis: rupture not disruption. Cell Microb 15, 190-199.
Krijnse Locker, J., and Schmid, S.L. (2013). Integrated electron microscopy: super-duper resolution. PLoS Biol 11 ppe1001639.
Miller, S., and Krijnse-Locker, J. (2008). Modification of intracellular membrane structures for virus replication. Nat Rev Microbiol 6, 363-374.
Muller, B., and Krijnse-Locker, J. (2014). Imaging of HIV assembly and release. Methods Mol Biol 1087, 167-184.
Suarez, C., Hoppe, S., Penard, E., Walther, P., and Krijnse Locker, J. (2017). Vaccinia Virus A11 Is Required for Membrane Rupture and Viral Membrane Assembly. Cellular microbiology.
Welsch, S., Keppler, O.T., Habermann, A., Allespach, I., Krijnse Locker, J., and Krausslich, H.-G. (2007). The primary site of HIV-1 budding in infected primary macrophages is the plasma membrane. . PLOS pathogen 3, 1-11.
Welsch, S., Miller, S., Romero-Brey, I., Merz, A., Bleck, C.K.E., Walther, P., Fuller, S.D., Antony, C., Krijnse-Locker, J., and Bartenschlager, R. (2009). Composition and three-dimensional architecture of the dengue virus replication and assembly sites. Cell Host & Microbe 5, 365-375.