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  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
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Scientific Fields
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About

Jacomine Krijnse Locker  heads the Ultra-structural bio-imaging unit (UBI) at the Institut Pasteur in Paris. She obtained her PhD in Utrecht, the Netherlands, on the cell biology of viruses. She specialized on EM-techniques at the European Molecular Biology laboratory, Heidelberg, Germany and subsequently set-up the EM facility at the Heidelberg University. In May 2015 she accepted to head the EM-platform at Pasteur Institute. Her main research interest is to study how viruses interact with cells to better understand how to treat the disease they cause.

 

Projects

CV

Research

The research group of the UBI studies membrane assembly of viruses, focusing predominantly on large DNA viruses. Using tools collected over the years, we use virus-infected cells to develop advanced EM-embedding and imaging methods that can be applied by the users of our platform. We put a particular emphasis on the development on two aspects in EM. The development of high-resolution cryo-EM methods to determine the structure of viral membrane-associated proteins in vitro and in situ. We also wish to broaden our portfolio of correlated- light and electron microscopy methods. For both we develop workflows that involve sample preparation, semi-automated imaging and image processing. We particularly welcome the input of autonomous users that can complement our efforts along their biological questions.   

Selected publications

Chlanda, P., Carbajal, M.A., Cyrklaff, M., Griffiths, G., and Krijnse Locker, J. (2009). Membrane rupture generates single open membrane sheets during vaccinia virus assembly. Cell Host & Microbe 6, 81-90.

Chlanda, P., and Krijnse Locker, J. (2017). The sleeping beauty kissed awake: new methods in electron microscopy to study cellular membranes. Biochem J 474, 1041-1053.

Krijnse Locker, J., Chlanda, P., Sachenheimer, T., and Brügger, B. (2013). poxvirus membrane biogenesis: rupture not disruption. Cell Microb 15, 190-199.

Krijnse Locker, J., and Schmid, S.L. (2013). Integrated electron microscopy: super-duper resolution. PLoS Biol 11 ppe1001639.

Miller, S., and Krijnse-Locker, J. (2008). Modification of intracellular membrane structures for virus replication. Nat Rev Microbiol 6, 363-374.

Muller, B., and Krijnse-Locker, J. (2014). Imaging of HIV assembly and release. Methods Mol Biol 1087, 167-184.

Suarez, C., Hoppe, S., Penard, E., Walther, P., and Krijnse Locker, J. (2017). Vaccinia Virus A11 Is Required for Membrane Rupture and Viral Membrane Assembly. Cellular microbiology.

Welsch, S., Keppler, O.T., Habermann, A., Allespach, I., Krijnse Locker, J., and Krausslich, H.-G. (2007). The primary site of HIV-1 budding in infected primary macrophages is the plasma membrane. . PLOS pathogen 3, 1-11.

Welsch, S., Miller, S., Romero-Brey, I., Merz, A., Bleck, C.K.E., Walther, P., Fuller, S.D., Antony, C., Krijnse-Locker, J., and Bartenschlager, R. (2009). Composition and three-dimensional architecture of the dengue virus replication and assembly sites. Cell Host & Microbe 5, 365-375.

Publications

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