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© Sandrine Etienne-Manneville
Photo prise à l'avant (dans la protrusion) d'astrocytes primaires de rat en migration. Marquage par immunofluorescence montrant en rouge, p150 Glued, une protéine associée aux extrémités 'plus' des microtubules et en vert la tubuline des microtubules. La photographie montre l'accumulation de p150 Glued à l'avant des cellules en migration, où la protéine pourrait participer à l'ancrage des microtubules à la membrane plasmique. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaires.
Member: Post-doc - Alumni

Hazel Silistre

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About

I’ve started my post-doctoral position at Institut Pasteur in the unit of Biochemistry of Macromolecular Interactions in January 2017. I am investigating the role of the ExoY nucleotidyl cyclase toxin which is one of the four effector proteins delivered to the host cells by the type III secretion system in Pseudomonas aeruginosa infections utilising the international reference panel of Pseudomons aeruginosa (de Soyza et al., 2013) and techniques such as in vitro nucletidyl cyclase activity assay, cellular infection models, microscopy, qPCR, Western Blotting and ELISA under the supervision of Dr. Undine Mechold and Prof. Daniel Ladant. Previously, I investigated the regulatory role of the RNA-binding protein RsmN in Pseudomonas aeruginosa virulence in phenotypic assays such as motiliy, biofilm formation, protease, elastase and pyocyanin assays and by utilising RNASeq during my PhD at the University of Nottingham, U.K., under the supervision of Prof. Paul Williams, Prof. Miguel Càmara and Prof. Stephan Heeb. I’ve completed my undergraduate studies -during which I’ve had internships at the Max Planck Institute for Biophysical Chemistry in Germany and Texas Tech University Health Sciences Center in the U.S.A.- in Biochemistry and Cell Biology at Jacobs University in Bremen, Germany. During my internship in Texas, I also investigated the contribution of quorum sening to the virulence of Pseudomonas aeruginosa in chronic and acute murine infection models under the supervision of Prof. Kendra Rumbaugh.

 

Publications

  • Romero, M., Silistre, H., Lovelock, L., Wright, V., Chan, k.-G., Hong, K.-W., Williams, P., Camara, M., Heeb, S. (submitted, Feb 2017)
    Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN
  • Morris, E. R., Hall, G., Li, C., Heeb, S., Kulkarni, R. V., Lovelock, L., Silistre, H., Messina, M., Camara, M., Emsley, J., Williams, P., and Searle, M. S. (2013) Structure 21, 1659-1671
    Structural Rearrangement in an RsmA/CsrA Ortholog of Pseudomonas aeruginosa
    Creates a Dimeric RNA-Binding Protein, RsmN