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© Research
Member: Graduate Student - Alumni

Charlotte Bouquerel

Scientific Fields
Diseases
Organisms
Applications
Technique

About

Charlotte BOUQUEREL is a biomedical engineering student. She obtained her Master Degree in Microfluidics at Pierre Gilles de Gennes Institute in 2017-2018 and specialized in Biomaterials at Polytechnique Montreal in 2018-2019.

Since Jan. 2019, Charlotte joined the “Biomaterial and Microfluidic” platform of  Pasteur Institute. She works with Samy Gobaa on her final project relying on laser photoablation of soft hydrogels to create vascular-like strutures within 3D cell cultures. Engineering blood vessels in vitro is an important tool for the discovery of drugs and therapeutic to treat many pathologies. The ability to fabricate multiple 3D microfluidics networks could significantly aid development on organ-on-chip as tissue vascularization remains a key barrier to the translation of engineered tissues into clinically relevant therapies.

Charlotte works in collaboration with Guillaume Dumenil on his project “Blood Vessels as a Target for Infection”, as one of the major challenges in bacterial infectious disease relates to infections taking place in the bloodstream (septicemia).

The experimental approach is to use photoablation as an innovative tool to reproduce the specific environment encountered by the bacteria in the bloodstream, and especially the specific geometry of the microcirculation. The perfusable microvessels created could allow further functional and structural modulations in response to biochemical and biophysical cues related to bacterial infection.

CV

EDUCATION

  • 2018-2019 : EXCHANGE SEMESTER – Polytechnique Montreal Biomaterials, Biomicrosytems, Regenerative Medicine
  • 2017-2018 : GAP YEAR – Master Degree at Pierre Gilles de Gennes Institute (ESPCI, ENS, ENSCP, UPMC) Microfluidics, Organ on chip, Rheology, Soft Matter, Biology
  • 2015-2019 : Engineering school  Telecom Physique Strasbourg – Degree in 2019 Information Technology (IT) in health
  • 2014-1015 : Second year of medical school – Claude Bernard Lyon 1 University
  • 2012-2014 : First year of medical school at Claude Bernard Lyon 1 Successful competitive examination « PACES »

WORK EXPERIENCE

  • Research intern @Biomaterials and Cartilage Laboratory Montreal (oct/2018 to dec/2018) : Developpment of a biomaterial associating chitosan polymer and growth factors to promote wound healing
  • Research intern @Singapore-MIT Alliance for Research and Technology (feb/2018 to july/2018) : Isolation of bladder cancer cells from urine using a spiral microfluidic device and downstream cellular characterization
  • Research intern @Biochemistry Laboratory (LBC) ESPCi Paris (june/2017 to sept/2017) : Quantifying the expression level of specific genes at the single cell level using reversetranscription (RT) and droplet based microfluidics