During her internship, Arianna will learn how to design conventional bi-allelic knockouts of the TTLL4B gene in Trypanosoma brucei cells (wild type and TTLL4A-/-). She will do this by obtaining the appropriate nucleic constructs, nucleofecting T. brucei cells, drug selection of mutant cells and their phenotypic analysis. This analysis will require use of several immunofluorescence and immunochemical, as well as dedicated softwares. More precisely, the glutamylation profile of microtubules issued from these mutants will be compared to the control (wild type or TTLL4A-/-). These experiments will help to decipher the role of the TTLL4B enzyme in the tubulin polyglutamylation process of T. brucei flagellum, its construction and function.
Bloodstream Trypanosoma brucei cell
Member: Undergraduate Student - Alumni