The Unit Lyssavirus Dynamics and Host Adaptation located at Institut Pasteur, Paris, France, a non-profit private foundation that contributes to research in biology, is looking for a
Post Doc position in RABIES Virus capping enzymes (ANR project RAB-Cap)
Mononegavirales such as Ebola and rabies virus are non-segmented negative strand RNA viruses causing important public health threats. Although prophylactic or post exposure vaccination is available against a limited set of Mononegavirales, we lack antiviral molecules for the treatment of non-vaccinated and/or people infected by rabies. It is therefore essential to identify conserved viral enzymes, playing a key role during the viral replication cycle, in order to design new antiviral strategies. The L protein of Mononegavirales is the main actor of virus replication, catalysing the replication, transcription and capping of the viral genome. Thus, these enzymatic activities embedded into the L protein are considered as good antiviral target.
The aim of this project is to decipher the Rabies virus capping machinery by characterizing the structure and the function of capping enzymes (PRNTase, N7- and 2’O-MTases) and to allow the identification of compounds inhibiting the MTase activity. The project will offer the opportunity to perform a complete study ranging from biochemical and structural analysis, and exploit reverse genetic tools in cell culture as well as subsequent in vivo translational applications using a mouse model. This project should contribute to a better understanding of the Mononegavirales transcription mechanism and potentially lead to the discovery of a viral strategy that couples transcription to immune evasion.
The funding was obtained in the frame of a grant obtained from the National Research Agency (ANR). The project will be performed at the Unit Lyssavirus Dynamics and Host Adaptation located at Institut Pasteur, Paris, France, under the supervision of Hervé Bourhy in collaboration with Etienne Decroly at the AFMB laboratory in Marseille, France (http://www.afmb.univ-mrs.fr/?lang=en) and Jonathan Grimes at the Division of Structural Biology, the Wellcome Trust Centre for Human Genetics, University of Oxford, UK (https://www.strubi.ox.ac.uk/research/jonathan-grimes).
The position is ideally suited for highly motivated and enthusiastic applicants with a PhD and a strong background in virology, biochemistry, molecular and cellular biology with an established publication record. Strong written and oral communications skills in english are required.
Experience in animal (mice model) experiments is encouraged.
- Additional Information :
Dead line for submission:October, 20th 2017
Start date : available from now (duration 2 years)
Location : Paris, France
Applicants should send their CV, a list of publication, a summary of their previous research activities (≤3 pages), a personnal statement and the names of three referees to the following e-mail address : firstname.lastname@example.org and use the following reference in the subject of the message: PostDoc RAB-CAP.
Website of the unit: https://research.pasteur.fr/fr/team/lyssavirus-dynamics-and-host-adaptation/