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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Equipment

Surface Plasmon Resonance Proteon XPR36

Scientific Fields
Diseases
Organisms
Applications
Technique

Biorad • Proteon XPR36

The PFBMI has two microfluidic SPR instruments (Biacore T200, GE Healthcare, and ProteOn XPR36, BioRad) that allow the label-free analysis of molecular interactions in real time. To that avail, one of the interaction partners is immobilized, covalently or non-covalently, while the other partner is brought in contact with the sensorchip surface using a continuous buffer flow in a microfluidic setup. This technique is notably suited for the determination of the rates of association (kon) and dissociation (koff) between biological macromolecules. Our instruments are entirely automatable, which allows to perform large series of highly reproducible experiments.