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© Research
Equipment

BioAxial CODIM100

BioAxial • CODIM100

Presentation.

The CODIM 100 is a super-resolution microscope that specialized in imaging live-cell.

PastedGraphic-1

It is the fruit of a collaboration between the Imagopole and the BioAxial company, on the development of an entirely new approach to super-resolution imaging based upon conical diffraction. It is now a microscopy system, available commercially, the CODIM100.

Features.

While there already exist several super-resolution modalities, this system is distinguished by the unique combination of several performance features. In brief:

  • it is ideal for live-cell super-resolution imaging (even long term time-lapse imaging);
  • it works with any fluorescent sample without any special preparation;
  • it has a  good sensitivity even to weakly labelled samples.

Specifications.

It is attached to a normal laser scanning confocal microscope, which can be used as is. The typical usage is to image a sample with normal LSCM mode, then draw a ROI over the acquired image to rescan it in super-resolution. Here are some quick specifications of the super-resolution module:

  • Excitation lines: 488, 561 and 640nm for super-resolution & confocal imaging.
  • Objectives: 40x 0.95 air / 60x 1.2 water / 60x 1.49 oil.
  • Acquisition speed: a few seconds for a ROI of 25 µm2.
  • Labels: any that can be excited with the  wavelength mentioned above 
  • Sample prep: any confocal or live sample with 0.17mm glass bottom coverslip. No restrictions on mounting media for fixed samples.
  • XY resolution: 110 nm with 488nm laser/0.95 NA, 90 nm with 488 nm laser/1.20 NA
  • Very low excitation power: less than 1 µW/µm2.
  • Motorized XY stage.
  • Mounted on a Nikon TI-Eclipse stand with the Perfect Focus stabilization system.

Publications and resources.

The following paper describe the technology, its implementation, and quantifies its phototoxicity impact:

A description of the conical diffraction phenomenon on the BioAxial website: 

Several publications that exploits the conical diffraction phenomenon as a general tool for optics:

Images taken from collaborators posters:

CODIM100_example1

CODIM100_example2