The CODIM 100 is a super-resolution microscope that specialized in imaging live-cell.
It is the fruit of a collaboration between the Imagopole and the BioAxial company, on the development of an entirely new approach to super-resolution imaging based upon conical diffraction. It is now a microscopy system, available commercially, the CODIM100.
While there already exist several super-resolution modalities, this system is distinguished by the unique combination of several performance features. In brief:
- it is ideal for live-cell super-resolution imaging (even long term time-lapse imaging);
- it works with any fluorescent sample without any special preparation;
- it has a good sensitivity even to weakly labelled samples.
It is attached to a normal laser scanning confocal microscope, which can be used as is. The typical usage is to image a sample with normal LSCM mode, then draw a ROI over the acquired image to rescan it in super-resolution. Here are some quick specifications of the super-resolution module:
- Excitation lines: 488, 561 and 640nm for super-resolution & confocal imaging.
- Objectives: 40x 0.95 air / 60x 1.2 water / 60x 1.49 oil.
- Acquisition speed: a few seconds for a ROI of 25 µm2.
- Labels: any that can be excited with the wavelength mentioned above
- Sample prep: any confocal or live sample with 0.17mm glass bottom coverslip. No restrictions on mounting media for fixed samples.
- XY resolution: 110 nm with 488nm laser/0.95 NA, 90 nm with 488 nm laser/1.20 NA
- Very low excitation power: less than 1 µW/µm2.
- Motorized XY stage.
- Mounted on a Nikon TI-Eclipse stand with the Perfect Focus stabilization system.
Publications and resources.
The following paper describe the technology, its implementation, and quantifies its phototoxicity impact:
- Conical diffraction illumination opens the way for low phototoxicity super-resolution imaging, Julien Caron , Clément Fallet , Jean-Yves Tinevez , Lionel Moisan , Philippe Braitbart , Gabriel Y Sirat , Spencer L Shorte , Cell Adhesion & Migration, Vol. 8, Iss. 5, 2014.
A description of the conical diffraction phenomenon on the BioAxial website:
Several publications that exploits the conical diffraction phenomenon as a general tool for optics:
- A new method to achieve tens of nm axial super-localisation based on conical diffraction PSF shaping, C Fallet, M Dubois, JY Tinevez, S Oddos, J Caron, R Persson, SL Shorte, G Sirat – SPIE BiOS, 2015
- Conical diffraction as a versatile building block to implement new imaging modalities for superresolution in fluorescence microscopy, C Fallet, J Caron, S Oddos, JY Tinevez, L Moisan, G Sirat, P Braitbart, SL Shorte – SPIE NanoScience+ Engineering, 2014
Images taken from collaborators posters: